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Tamil Nadu Board 12th Standard Botany - Chapter 4: Book Back Answers and Solutions
This post covers the book back answers and solutions for Chapter 4 – Botany from the Tamil Nadu State Board 12th Standard Botany textbook. These detailed answers have been carefully prepared by our expert teachers at KalviTips.com.
We have explained each answer in a simple, easy-to-understand format, highlighting important points step by step under the relevant subtopics. Students are advised to read and memorize these subtopics thoroughly. Once you understand the main concepts, you’ll be able to connect other related points with real-life examples and confidently present them in your tests and exams.
By going through this material, you’ll gain a strong understanding of Botany Chapter 4 along with the corresponding book back questions and answers (PDF format).
Question Types Covered:
- 1 Mark Questions: Choose the correct answer, Fill in the blanks, Identify the correct statement, Match the following
- 2 Mark Questions: Answer briefly
- 3, 4, and 5 Mark Questions: Answer in detail
All answers are presented in a clear and student-friendly manner, focusing on key points to help you score full marks.
All the best, Class 12 students! Prepare well and aim for top scores. Thank you!
Unit 4 - Principles and Processes of Biotechnology
I. Multiple Choice Questions
1. Restriction enzymes are
a. Not always required in genetic engineering
b. Essential tools in genetic engineering
c. Nucleases that cleave DNA at specific sites
d. both b and c
Answer Key:
d. both b and c
2. Plasmids are
a. circular protein molecules
b. required by bacteria
c. tiny bacteria
d. confer resistance to antibiotics
Answer Key:
d. confer resistance to antibiotics
3. EcoRI cleaves DNA at
a. AGGGTT
b. GTATATC
c. GAATTC
d. TATAGC
Answer Key:
c. GAATTC
4. Genetic engineering is
a. making artificial genes.
b. hybridization of DNA of one organism to that of the others.
c. production of alcohol by using micro organisms.
d. making artificial limbs, diagnostic instruments such as ECG, EEG etc.,
Answer Key:
b. hybridization of DNA of one organism to that of the others.
5. Consider the following statements:
I. Recombinant DNA technology is popularly known as genetic engineering is a stream of biotechnology which deals with the manipulation of genetic materials by man invitro
II. pBR322 is the first artificial cloning vector developed in 1977 by Boliver and Rodriguez from E.coli plasmid
III. Restriction enzymes belongs to a class of enzymes called nucleases.
Choose the correct option regarding above statements
a. I & II
b. I & III
c. II & III
d. I,II & III
Answer Key:
d. I,II & III
6. The process of recombinant DNA technology has the following steps
I. amplication of the gene
II. Insertion of recombinant DNA into the host cells
III. Cutting of DNA at specific location using restriction enzyme .
IV. Isolation of genetic material (DNA) Pick out the correct sequence of step for recombinant DNA technology.
a. II, III, IV, I
b. IV, II, III, I
c. I, II, III, IV
d. IV, III, I, II
Answer Key:
d. IV, III, I, II
7. Which one of the following palindromic base sequence in DNA can be easily cut at about the middle by some particular restriction enzymes?
a. 5 CGTTCG 3 3 ATCGTA 5
b. 5 GATATG 3 3 CTACTA 5
c. 5 GAATTC 3 3 CTTAAG 5
d. 5 CACGTA 3 3 CTCAGT 5
Answer Key:
c. 5 GAATTC 3 3 CTTAAG 5
8. pBR 322, BR stands for
a. Plasmid Bacterial Recombination
b. Plasmid Bacterial Replication
c. Plasmid Boliver and Rodriguez
d. Plasmid Baltimore and Rodriguez
Answer Key:
c. Plasmid Boliver and Rodriguez
9. Match the following:
1 2 3 4
A) a b c d
B) c d b a
C) a c b d
D) c d a b
Answer Key:
D) c d a b
10. In which techniques Ethidium Bromide is used?
a. Southern Blotting techniques
b. Western Blotting techniques
c. Polymerase Chain Reaction
d. Agrose Gel Electroporosis
Answer Key:
d. Agrose Gel Electroporosis
11. Assertion : Agrobacterium tumifaciens is popular in genetic engineering because this bacteriumis associated with the root nodules of all cereals and pulse crops
Reason: A gene incorporated in the bacterial chromosomal genome gets atomatically transferred to the cross with which bacterium is associated.
a) Both assertion and reason are true. But reason is correct explanation of assertion.
b) Both assertion and reason are true. But reason is not correct explanation of assertion.
c) Assertion is true, but reason is false.
d) Assertion is false, but reason is true.
e) Both assertion and reason are false.
Answer Key:
e) Both assertion and reason are false.
12. Which one of the following is not correct statement.
a) Ti plasmid causes the bunchy top disease
b) Multiple cloning site is known as Polylinker
c) Non viral method transfection of Nucleic acid in cell
d) Polylactic acid is a kind of biodegradable and bioactive thermoplastic.
Answer Key:
a) Ti plasmid causes the bunchy top disease
13. An analysis of chromosomal DNA using the southern hybridisation technique does not use
a) Electrophoresis
b) Blotting
c) Autoradiography
d) Polymerase Chain Reaction
Answer Key:
d) Polymerase Chain Reaction
14. An antibiotic gene in a vector usually helps in the selection of
a) Competent cells
b) Transformed cells
c) Recombinant cells
d) None of the above
Answer Key:
b) Transformed cells
15. Some of the characteristics of Bt cotton are
a) Long fibre and resistant to aphids
b) Medium yield, long fibre and resistant to beetle pests
c) high yield and production of toxic protein crystals which kill dipteran pests.
d) High yield and resistant to ball worms
Answer Key:
d) High yield and resistant to ball worms
a. Not always required in genetic engineering
b. Essential tools in genetic engineering
c. Nucleases that cleave DNA at specific sites
d. both b and c
Answer Key:
d. both b and c
2. Plasmids are
a. circular protein molecules
b. required by bacteria
c. tiny bacteria
d. confer resistance to antibiotics
Answer Key:
d. confer resistance to antibiotics
3. EcoRI cleaves DNA at
a. AGGGTT
b. GTATATC
c. GAATTC
d. TATAGC
Answer Key:
c. GAATTC
4. Genetic engineering is
a. making artificial genes.
b. hybridization of DNA of one organism to that of the others.
c. production of alcohol by using micro organisms.
d. making artificial limbs, diagnostic instruments such as ECG, EEG etc.,
Answer Key:
b. hybridization of DNA of one organism to that of the others.
5. Consider the following statements:
I. Recombinant DNA technology is popularly known as genetic engineering is a stream of biotechnology which deals with the manipulation of genetic materials by man invitro
II. pBR322 is the first artificial cloning vector developed in 1977 by Boliver and Rodriguez from E.coli plasmid
III. Restriction enzymes belongs to a class of enzymes called nucleases.
Choose the correct option regarding above statements
a. I & II
b. I & III
c. II & III
d. I,II & III
Answer Key:
d. I,II & III
6. The process of recombinant DNA technology has the following steps
I. amplication of the gene
II. Insertion of recombinant DNA into the host cells
III. Cutting of DNA at specific location using restriction enzyme .
IV. Isolation of genetic material (DNA) Pick out the correct sequence of step for recombinant DNA technology.
a. II, III, IV, I
b. IV, II, III, I
c. I, II, III, IV
d. IV, III, I, II
Answer Key:
d. IV, III, I, II
7. Which one of the following palindromic base sequence in DNA can be easily cut at about the middle by some particular restriction enzymes?
a. 5 CGTTCG 3 3 ATCGTA 5
b. 5 GATATG 3 3 CTACTA 5
c. 5 GAATTC 3 3 CTTAAG 5
d. 5 CACGTA 3 3 CTCAGT 5
Answer Key:
c. 5 GAATTC 3 3 CTTAAG 5
8. pBR 322, BR stands for
a. Plasmid Bacterial Recombination
b. Plasmid Bacterial Replication
c. Plasmid Boliver and Rodriguez
d. Plasmid Baltimore and Rodriguez
Answer Key:
c. Plasmid Boliver and Rodriguez
9. Match the following:
|
Column A
|
Column B
|
|
1 Exonuclease
|
a. add or remove phosphate
|
|
2 Endonuclease
|
b. binding the DNA fragments
|
|
3 Alkaline Phosphatase
|
c. cut the DNA at terminus
|
|
4 Ligase
|
d. cut the DNA at middle
|
A) a b c d
B) c d b a
C) a c b d
D) c d a b
Answer Key:
D) c d a b
10. In which techniques Ethidium Bromide is used?
a. Southern Blotting techniques
b. Western Blotting techniques
c. Polymerase Chain Reaction
d. Agrose Gel Electroporosis
Answer Key:
d. Agrose Gel Electroporosis
11. Assertion : Agrobacterium tumifaciens is popular in genetic engineering because this bacteriumis associated with the root nodules of all cereals and pulse crops
Reason: A gene incorporated in the bacterial chromosomal genome gets atomatically transferred to the cross with which bacterium is associated.
a) Both assertion and reason are true. But reason is correct explanation of assertion.
b) Both assertion and reason are true. But reason is not correct explanation of assertion.
c) Assertion is true, but reason is false.
d) Assertion is false, but reason is true.
e) Both assertion and reason are false.
Answer Key:
e) Both assertion and reason are false.
12. Which one of the following is not correct statement.
a) Ti plasmid causes the bunchy top disease
b) Multiple cloning site is known as Polylinker
c) Non viral method transfection of Nucleic acid in cell
d) Polylactic acid is a kind of biodegradable and bioactive thermoplastic.
Answer Key:
a) Ti plasmid causes the bunchy top disease
13. An analysis of chromosomal DNA using the southern hybridisation technique does not use
a) Electrophoresis
b) Blotting
c) Autoradiography
d) Polymerase Chain Reaction
Answer Key:
d) Polymerase Chain Reaction
14. An antibiotic gene in a vector usually helps in the selection of
a) Competent cells
b) Transformed cells
c) Recombinant cells
d) None of the above
Answer Key:
b) Transformed cells
15. Some of the characteristics of Bt cotton are
a) Long fibre and resistant to aphids
b) Medium yield, long fibre and resistant to beetle pests
c) high yield and production of toxic protein crystals which kill dipteran pests.
d) High yield and resistant to ball worms
Answer Key:
d) High yield and resistant to ball worms
II. Short Answer Questions
12th bio botanyIII. Long Answer Questions
12th bio botanyIV. Exercise
12thbio botany
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